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Its name comes from the Latin vimentum which refers to an array of flexible rods. Immunofluorescence staining of HeLa Cells with antibody to reveal vimentin containing intermediate filaments in green and antibody to LAMP1 to reveal lysosomes in red. Vimentin is found in all types of sarcomas and lymphomas. Positive staining for vimentin is seen in most cells of fibrosarcomas, liposarcomas, malignant fibrous histocytomas, angiosarcomas, chondrosarcomas and lymphomas. All melanomas and Schwannomas are strongly vimentin-positive.

Vimentin stain

For full image see PMID 28665955. Flow Cytometry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) Myofibroblasts usually stain for the intermediate filament vimentin which is a general mesenchymal marker and "alpha smooth muscle actin" (human gene = ACTA2) and for palladin, which is a cytoskeletal actin scaffold protein. Mesenchymal and endothelial cells usually stain vimentin positive and thus act as a measure of internal quality control in immunoreactivity. Absence of immunostaining in these areas often indicates that there is significant damage to tissue antigens and loss of structural architecture. We retrospectively reviewed the pathology archives in our institution for all specimens with vimentin stain between 2000 and 2016.

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Despite the abundance of clinical data linking vimentin to poor patient outcome, it is unclear if vimentin is required for metastasis or is a correlative biomarker. We developed a novel genetically engineered Vimentin is a fairly widespread cytoskeletal component encoding for intermediate filaments.

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It marks most soft tissue lesions and malignant melanoma. It is usually negative in carcinomas. Vimentin Antibody Staining Protocol for Immunohistochemistry . Description: This monoclonal antibody reacts with the intermediate filament protein vimentin present in cells of mesenchymal origin. Broad interspecies cross-reactivity has been observed with human, rat, and chicken vimentin. a Representative IHC stainings for vimentin and Pax-5 in nt-FL and corresponding s-FL/s-tFL. Stains were performed on consecutive slides.

Cells were grown on chamber slides and fixed with formaldehyde prior to staining. 2019-03-18 · Vimentin is widely used as a routine IHC biomarker for phenotyping neoplasia of mesenchymal and melanocytic origin, and is also expressed in a smaller range of epithelial tumors. Common stain used in panels for mesenchymal markers Confirms myogenic origin of tissue / tumors Helps differentiate smooth muscle tumors (desmin+) from GIST (c-kit+, almost always desmin-) In serous effusion, more common in reactive mesothelial cells (84%) than mesothelioma (8%) or carcinoma (2%, Am J Surg Pathol 2001;25:1405) Mouse monoclonal anti-human cytokeratin (AE1/AE3) and rabbit monoclonal anti-human vimentin (SP20) antibodies were used as primary antibodies, followed by staining with Alexa Fluor-conjugated secondary antibodies. Staining using the RMIF method was compared with enzyme-based ICC staining.
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Vimentin antibody detects vimentin, a type III intermediate filament protein with a predicted molecular weight of 54 kDa. Vimentin is highly expressed in cells of mesodermal origin as well as in some ectodermal cells, and is often used as a marker of mesenchymal stem cells. indolyl phosphate toluidine salt–phosphatase substrate. A positive reaction stains the membrane of the cells gray to black.

Immunofluorescent analysis of Vimentin using Vimentin Monoclonal antibody (J144) (Product # MA3-745) shows staining in HeLa cells. Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining.
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Positive staining for vimentin is seen in most cells of fibrosarcomas, liposarcomas, malignant fibrous histocytomas, angiosarcomas, chondrosarcomas and lymphomas. All melanomas and Schwannomas are strongly vimentin-positive. Vimentin is one of the 5 major intermediate filaments found in cells (cytokeratin, vimentin, neurofilament, desmin, and glial fibrillary acid protein [GFAP]). It was classically thought to be limited to mesenchymal cells only, and in the early days of immunohistochemistry, it was thought to be a specific marker for sarcomas and other mesenchymal neoplasms. Because tissue culture studies have suggested that mesothelial cells might produce large amounts of vimentin, I stained eight mesotheliomas (two fixed in alcohol) for vimentin using the Gown and Vogel monoclonal antibody 43 beta E8. Vimentin Antibody Staining Protocol for Immunohistochemistry Description: Vimentin is an intermediate filament that is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium.